製品の概要

  • 製品名Anti-p53R2 antibody
    p53R2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p53R2
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GDPERPEAAGLDQDER

    , corresponding to amino acids 2-17 of Human p53R2.

  • ポジティブ・コントロール
    • A431 or 3T3 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab8105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).Can be blocked with p53R2 peptide (ab6237).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 18997010

ターゲット情報

  • 機能Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
  • 組織特異性Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
  • パスウェイGenetic information processing; DNA replication.
  • 関連疾患Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
    Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • 配列類似性Belongs to the ribonucleoside diphosphate reductase small chain family.
  • 細胞内局在Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686M05248 antibody
    • MGC102856 antibody
    • MGC42116 antibody
    • MTDPS8A antibody
    • MTDPS8B antibody
    • p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
    • p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
    • P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
    • p53 R2 antibody
    • p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
    • p53R2 antibody
    • Ribonucleoside diphosphate reductase M2 subunit B antibody
    • Ribonucleoside-diphosphate reductase subunit M2 B antibody
    • Ribonucleotide reductase M2 B (TP53 inducible) antibody
    • Ribonucleotide reductase M2 B antibody
    • Ribonucleotide reductase small subunit like 2 p53 inducible antibody
    • RIR2B_HUMAN antibody
    • RRM 2B antibody
    • RRM2B antibody
    • TP53 inducible ribonucleotide reductase M2 B antibody
    • TP53-inducible ribonucleotide reductase M2 B antibody
    see all

Anti-p53R2 antibody 画像

  • Anti-p53R2 antibody (ab8105) at 1/1000 dilution + U2OS whole cell lysate treated with UV irradiation.

    Secondary
    Goat anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa (possible cross reactivity).

    Exposure time : 1 minute

    Image was taken from an anonymously submitted Abreview

    Blot was incubated for one hour in 5% milk for blocking, and incubated in primary antibody for 16 hours.
  • All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml

    Lane 1 : A431 cell lysate
    Lane 2 : 3T3 cell lysate with absence of blocking peptide
    Lane 3 : 3T3 cell lysate with presence of blocking peptide


    Predicted band size : 41 kDa
  • ab8105 at 1µg/ml staining p53R2 in lung tissue by IHC

  • ICC/IF image of ab8105 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8105, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of p53R2 in Human Lung cells using ab8105 at 20 ug/ml.

Anti-p53R2 antibody (ab8105) 使用論文

This product has been referenced in:
  • Kuny CV & Kalejta RF Human Cytomegalovirus Can Procure Deoxyribonucleotides for Viral DNA Replication in the Absence of Retinoblastoma Protein Phosphorylation. J Virol 90:8634-43 (2016). Read more (PubMed: 27440891) »
  • Graindorge D  et al. Singlet Oxygen-Mediated Oxidation during UVA Radiation Alters the Dynamic of Genomic DNA Replication. PLoS One 10:e0140645 (2015). WB ; Human . Read more (PubMed: 26485711) »

See all 14 Publications for this product

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Sep 01 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"