製品の概要

  • 製品名Anti-p53 BP3 antibody
    p53 BP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to p53 BP3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Fusion protein:

    NKRSRSRESSSRPRARKDKKRSRTRDSSWSRRAQTLSLSSGSTSRSRSRS SDHGKRRSRSRNRDRYYLRNNYGSKYKWEYTYYSRNKDRDGYESSYRRRT

    , corresponding to amino acids 625/724 of Mouse p53 BP3

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 119 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
  • 組織特異性Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.
  • 関連疾患Defects in TOPORS are the cause of retinitis pigmentosa type 31 (RP31) [MIM:609923]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP31 inheritance is autosomal dominant.
  • 配列類似性Contains 1 RING-type zinc finger.
  • 翻訳後修飾Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.
    Sumoylated.
  • 細胞内局在Nucleus. Nucleus > PML body. Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW105885 antibody
    • E3 ubiquitin protein ligase Topors antibody
    • E3 ubiquitin-protein ligase Topors antibody
    • LUN antibody
    • p53 binding protein 3 antibody
    • p53 BP3/LUN antibody
    • p53-binding protein 3 antibody
    • p53BP3 antibody
    • p53BP3/LUN antibody
    • Retinitis pigmentosa 31 antibody
    • RP31 antibody
    • SUMO1 protein E3 ligase Topors antibody
    • SUMO1-protein E3 ligase Topors antibody
    • Topoisomerase I binding arginine/serine rich antibody
    • Topoisomerase I binding arginine/serine rich protein antibody
    • Topoisomerase I binding protein antibody
    • Topoisomerase I binding RING finger protein antibody
    • Topoisomerase I-binding arginine/serine-rich protein antibody
    • Topoisomerase I-binding RING finger protein antibody
    • Topoisomerase I-binding RS protein antibody
    • Topors antibody
    • TOPRS_HUMAN antibody
    • TP53BPL antibody
    • Tumor protein p53 binding protein antibody
    • Tumor suppressor p53 binding protein 3 antibody
    • Tumor suppressor p53-binding protein 3 antibody
    see all

Anti-p53 BP3 antibody 画像

  • All lanes : Anti-p53 BP3 antibody (ab21985) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 119 kDa

Anti-p53 BP3 antibody (ab21985) 使用論文

ab21985 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab21985.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"