Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, the antibody produced a nuclear-enriched focal staining pattern in interphase nuclei. MeOH-fixed samples were also evaluated, but failed to produce the expected staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jan 16 2017