Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
20 µg
Specification
HepG2
Treatment
25 nM TCDD 24h then 75 µM Etoposide for 24h
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
I-block as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: I-block
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit T2191
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/20000
Detection
Detection method
CDP star
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 53 kDa
Additional data
PubMed ID
20299546: View
Additional Notes
Legend of figure:
1. 0.15% DMSO
2. 25nM TCDD
3. 75 µM Etoposide
4. 75 µM Etoposide +25nM TCDD
1. 0.15% DMSO
2. 25nM TCDD
3. 75 µM Etoposide
4. 75 µM Etoposide +25nM TCDD
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. M Aggerbeck
Verified customer
投稿 Nov 26 2010