製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to P4HA3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to internal sequence amino acids 130-396 of Human P4HA3 (BC117333).

  • ポジティブ・コントロール
    • Human fetal kidney & liver lysates, Human fetal liver tissue.

法規制情報

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • 組織特異性
    Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
  • 配列類似性
    Belongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • 翻訳後修飾
    N-glycosylation plays no role in the catalytic activity.
  • 細胞内局在
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • 4-PH alpha-3 antibody
    • P4ha3 antibody
    • P4HA3_HUMAN antibody
    • Procollagen-proline antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase, alpha subunit, isoform 3 antibody
    • Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide III antibody
    • Prolyl 4-hydroxylase, alpha-3 subunit antibody
    see all

Anti-P4HA3 antibody 画像

  • All lanes : Anti-P4HA3 antibody (ab101657) at 1/500 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate


    Predicted band size : 61 kDa
  • ab101657 at 1/100 dilution staining P4HA3 in Human fetal liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Anti-P4HA3 antibody (ab101657) 使用論文

This product has been referenced in:
  • Olivares O  et al. Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. Nat Commun 8:16031 (2017). WB, IHC . Read more (PubMed: 28685754) »
  • Akamatsu T  et al. Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences. Fibrogenesis Tissue Repair 6:15 (2013). IHC-P . Read more (PubMed: 23927729) »

See all 2 Publications for this product

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There are currently no Abreviews or Questions for ab101657.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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