製品の概要

  • 製品名Anti-P4HA2 antibody
    P4HA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to P4HA2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • 配列類似性Belongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • 細胞内局在Endoplasmic reticulum lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • 4 PH alpha 2 antibody
    • 4-PH alpha-2 antibody
    • C-P4Halpha(II) antibody
    • collagen prolyl 4-hydroxylase alpha(II) antibody
    • P4HA2 antibody
    • P4HA2_HUMAN antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2 antibody
    • Procollagen-proline antibody
    • procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • Prolyl 4 hydroxylase alpha 2 subunit antibody
    • Prolyl 4 hydroxylase subunit alpha 2 [Precursor] antibody
    • Prolyl 4-hydroxylase subunit alpha-2 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide II antibody
    see all

Anti-P4HA2 antibody 画像

  • All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 28 kDa,33 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 35 kDa (possible degradation product).

    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-P4HA2 antibody (ab70887) 使用論文

ab70887 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat cell lines)
Loading amount 20 µg
Specification Rat cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Feb 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"