Anti-p38 (phospho T180 + Y182) 抗体 (ab4822)

製品の概要

  • 製品名Anti-p38 (phospho T180 + Y182) antibody
    p38 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p38 (phospho T180 + Y182)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog, Carp, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human p38 (phospho T180 + Y182). p38 is dually phosphorylated and therefore fully activated by MEK3 and MEK6 on threonine 180 and tyrosine 182 within the activation loop.
    Database link: Q16539
    (Peptide available as ab5253)

  • ポジティブ・コントロール
    • HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free. PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 38 kDa.

ターゲット情報

  • 機能Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • 組織特異性Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Anti-p38 (phospho T180 + Y182) antibody 画像



  • Predicted band size : 38 kDa
    Peptide Competition: Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide

Anti-p38 (phospho T180 + Y182) antibody (ab4822) 使用論文

This product has been referenced in:
  • Kang K  et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep 6:22632 (2016). WB . Read more (PubMed: 26961159) »
  • Pan W  et al. Resveratrol Protects against TNF-a-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK. PLoS One 11:e0147034 (2016). Human . Read more (PubMed: 26799794) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Mouse Tissue sections (retina)
Specification retina
Permeabilization Yes - triton x100
Fixative Paraformaldehyde
Username

Dr. Minzhong Yu

Verified customer

投稿 Feb 27 2015

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

S...

Read More

Thank you for contacting us. I am sorry to hear the customer has been having problems with this antibody. I think the right band is being detected at ~55 kDa. I would like to suggest some tips that might help improve the results: - Decr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount 10 µg
Specification Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (skeletal muscle)
Loading amount 20 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2008

Thank you for your enquiry and patience. The concentration for this batch is 0.125 mg/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"