Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 抗体 [Y122] (ab32142)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] -
製品の詳細
Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14 -
由来種
Rabbit -
特異性
This antibody recognises p38. It is predicted to react with splice isoform CSBP1 according to sequence homology. Mouse cross reactivity has been tested by WB and IHC, Rat cross reactivity by WB only. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Hela, Jurkat, HEK-293T, K562 and MCF7 cell lyastes. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
Y122 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab193897)
- Alexa Fluor® 647 Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab196646)
- Alexa Fluor® 594 Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab214842)
- Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free (ab227995)
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32142の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
p38 alpha/MAPK14: Cytoplasm. Nucleus. -
参照データベース
- Entrez Gene: 1432 Human
- Entrez Gene: 5600 Human
- Entrez Gene: 19094 Mouse
- Entrez Gene: 26416 Mouse
- Entrez Gene: 689314 Rat
- Entrez Gene: 81649 Rat
- Omim: 600289 Human
- Omim: 602898 Human
see all
画像
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All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution
Lane 1 : MAPK11 recombinant (ab117219)
Lane 2 : MAPK12 recombinant (ab117221)
Lane 3 : MAPK13 recombinant (ab113869)
Lane 4 : MAPK14 (p38) recombinant (ab82188)
Lysates/proteins at 0.5 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Green - ab32142 observed at 43 kDa.
ab32142 was shown to react with Anti-p38 antibody [Y122] in Western blot. Membranes were blocked in 100% Licor before incubation with ab32142 and overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution
Lane 1 :Recombinant Human p38 beta/MAPK11 protein (ab117219)
Lane 2 :Recombinant Human p38 gamma/MAPK12 protein (ab117221)
Lane 3 :Recombinant Human p38 delta/MAPK13 protein (ab113869)
Lane 4 :Recombinant Human p38 alpha/MAPK14 protein (ab82188)
Predicted band size: 41 kDa -
All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MAPK14 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : p38 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : p38 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
This western blot image is a comparison between ab32142 and a competitor's top cited rabbit polyclonal antibody.
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All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
10 ug protein per lane
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (42)
ab32142 は 42 報の論文で使用されています。
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Zhuang Q et al. Knockdown of circ-RAD23B inhibits non-small cell lung cancer progression via the miR-142-3p/MAP4K3 axis. Thorac Cancer 13:750-760 (2022). PubMed: 35106926
- Song XY et al. Cangxitongbi capsules protect the articular cartilage in the rat knee through the long non-coding RNA HOTAIR/p38MAPK pathway. Ann Transl Med 10:23 (2022). PubMed: 35242868
- Zhang Y et al. FimH confers mannose-targeting ability to Bacillus Calmette-Guerin for improved immunotherapy in bladder cancer. J Immunother Cancer 10:N/A (2022). PubMed: 35361729
- Xu W et al. tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway. Front Oncol 11:733763 (2021). PubMed: 34497772