製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to p38
  • 特異性
    ab27986 detects total p38 MAP kinase. Does not cross react with either JNK/SAPK or p44/42 MAP kinase.
  • アプリケーション
    適用あり: WB, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)(Peptide available as ab53314.)

  • ポジティブ・コントロール
    • Jurkat cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).Can be blocked with p38 peptide (ab53314).
IP Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • 組織特異性
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメイン
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

画像

  • Anti-p38 antibody (ab27986) at 1/500 dilution + mouse retina whole tissue lysate at 40 µg

    Secondary
    Polyclonal Goat anti-rabbit IgG-HRP at 1/200 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 hours

    This image is courtesy of an anonymous abreview.

    Blocking buffer: 3% BSA

    See Abreview

  • Anti-p38 antibody (ab27986) at 2 µg/ml (Jurkat cell lysate)

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Zhu Y  et al. a- and ß-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage. Exp Ther Med 13:1143-1150 (2017). WB ; Human . Read more (PubMed: 28450955) »
  • Liang RN  et al. Ping-Chong-Jiang-Ni Formula Induces Apoptosis and Inhibits Proliferation of Human Ectopic Endometrial Stromal Cells in Endometriosis via the Activation of JNK Signaling Pathway. Evid Based Complement Alternat Med 2017:6489427 (2017). WB ; Rat . Read more (PubMed: 28656053) »

See all 9 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (retina)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Jul 06 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Cell (retina)
Specification
retina
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
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Verified customer

投稿 Jan 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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