Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Tissue lysate - whole (Mouse whole embryo (E10.5) extract)
Specification
Mouse whole embryo (E10.5) extract
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T with 5% milk powder
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 38 kDa
Exposure
5 second(s)
Positive control
E10.5 mouse whole embryo extracts were prepared in RIPA lysis buffer and loaded for western blot analysis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 25 2014