製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to p35
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • 免疫原

    Recombinant tagged fragment: CRDVISSEVG SDHELQAVLL TCLYLSYSYM GNEISYPLKP FLVESCKEAF WDRCLSVINL MSSKMLQINA DPHYFTQVFS DLKNESGQED KKRLLLGLDR, corresponding to amino acids 208-308 of Human p35

  • ポジティブ・コントロール
    • p35 transfected 293T cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab10570 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 組織特異性
    Brain and neuron specific.
  • 配列類似性
    Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻訳後修飾
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 細胞内局在
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all

画像

  • All lanes : Anti-p35 antibody (ab66064) at 1 µg/ml

    Lane 1 : p35 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • IHC image of ab66064 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66064, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Xie W  et al. Phosphorylation of kinase insert domain receptor by cyclin-dependent kinase 5 at serine 229 is associated with invasive behavior and poor prognosis in prolactin pituitary adenomas. Oncotarget 7:50883-50894 (2016). IHC-P ; Human . Read more (PubMed: 27438154) »
  • Hou C  et al. Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med 38:713-20 (2016). WB ; Mouse . Read more (PubMed: 27460435) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Sample
Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
Specification
human iPS cell derived neurons (healthy/diseased)
Treatment
quinolinic acid 72h (and non treated) 500nM
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Caudate Putamen)
Loading amount
70 µg
Specification
Brain Caudate Putamen
Treatment
THC or Vehicle for 14 days
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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