製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to p35
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Cat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 255-304 (EAFWDRCLSV INLMSSKMLQ INADPHYFTQ VFSDLKNESG QEDKKRLLLG) of Mouse p35 (NP_034001.1).

  • ポジティブ・コントロール
    • Mouse pancreas lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 組織特異性
    Brain and neuron specific.
  • 配列類似性
    Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻訳後修飾
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 細胞内局在
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD5R1_HUMAN antibody
    • CDK5 activator 1 antibody
    • CDK5P35 antibody
    • CDK5R antibody
    • CDK5R1 antibody
    • Cyclin dependent kinase 5 regulatory subunit 1 antibody
    • Cyclin-dependent kinase 5 activator 1 antibody
    • Cyclin-dependent kinase 5 regulatory subunit 1 antibody
    • NCK 5A antibody
    • Neuronal CDK5 activator antibody
    • p23 antibody
    • p25 antibody
    • p35 antibody
    • Regulatory partner for CDK5 kinase antibody
    • Tau protein kinase II 23 kDa subunit antibody
    • TPKII regulatory subunit antibody
    see all

画像

  • Anti-p35 antibody (ab123048) at 1 µg/ml + Mouse pancreas lysate at 10 µg

    Predicted band size : 34 kDa

プロトコール

参考文献

ab123048 has not yet been referenced specifically in any publications.

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