Goat polyclonal to P2RX4
Rat, Cow, Dog
Synthetic peptide with sequence C-YREKKYKYVEDYEQ, from the C terminus of the human P2RX4 protein (NP_002551.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
ab77434 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Belongs to the P2X receptor family.
Information by UniProt
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
Western blot - Anti-P2RX4 antibody (ab77434)
All lanes :
Anti-P2RX4 antibody (ab77434) at 1 µg/mlLane 1 :
Human kidney lysate (in RIPA buffer)Lane 2 :
Human liver lysate (in RIPA buffer)Lane 3 :
Rat kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.Predicted band size :
43 kDaObserved band size :
60 kDa (why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"