Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
I needed to use a micropolymer HRP kit to obtain this intensity of immunostaining as I could not achieve a similar intensity of positivity on human tissues, under the same conditions ( compare with results on rat/mouse/marmoset sections, using a std ABCpx detection system).
There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.
There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 02 2010