The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 21 kDa.
The human ras gene family consists of three identified members which encode proteins of 21 kDa. Human c-H-ras and c-K-ras are the cellular homologs of v-Hand v-K-ras originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. The third family member is designated c-N-ras. Normal cellular ras genes are referred to as protooncogenes and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61. Such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis remains to be determined.
IHC image of ab86696 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86696, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.