Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain and Spleen)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Brain and Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-rabbit IgG HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminiscence
Exposure
30 second(s)
Bands
Specific: 16 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ester Canto Puig
Verified customer
投稿 Jul 04 2016