Anti-p107 (phospho T369) 抗体 (ab111348)

製品の概要

  • 製品名
    Anti-p107 (phospho T369) antibody
    p107 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p107 (phospho T369)
  • 特異性
    ab111348 detects endogenous levels of p107 only when phosphorylated at threonine 369.
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from Human p107 around the phosphorylation site of threonine 369 (P-S-TP-P-L).

  • ポジティブ・コントロール
    • Human lymph node tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
  • 配列類似性
    Belongs to the retinoblastoma protein (RB) family.
  • 翻訳後修飾
    Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 107 kDa retinoblastoma-associated protein antibody
    • Cellular protein 107 antibody
    • Cellular protein p107 antibody
    • CP107 antibody
    • MGC40006 antibody
    • p107 antibody
    • PRB1 antibody
    • RBL1 antibody
    • RBL1_HUMAN antibody
    • Retinoblastoma like protein 1 antibody
    • retinoblastoma-like 1 (p107) antibody
    • Retinoblastoma-like protein 1 antibody
    see all

画像

  • ab111348 at 1/50 dilution staining p107 in paraffin-embedded Human Lymph node tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

プロトコール

参考文献

ab111348 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Inner Ears)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Mouse Inner Ears
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Shikha Tarang

Verified customer

投稿 Aug 17 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (6% gel)
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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