製品の概要

  • 製品名Anti-P Glycoprotein antibody [F4]
    P Glycoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F4] to P Glycoprotein
  • 特異性ab80594 detects soluble P glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P glycoprotein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human, Chinese Hamster
  • 免疫原

    Human and hamster drug-resistant viable cells followed by crude plasma membranes.

  • エピトープExtracellular amino terminal half. This epitope is resistant to formalin fixation and periodate oxidation.
  • ポジティブ・コントロール
    • Drug resistant cells. Human kidney or breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
Flow Cyt
ICC/IF
  • 追加情報Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 141 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • 組織特異性Expressed in liver, kidney, small intestine and brain.
    • 関連疾患Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • 配列類似性Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABC20 antibody
      • ABCB1 antibody
      • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
      • ATP-binding cassette sub-family B member 1 antibody
      • CD243 antibody
      • CLCS antibody
      • Colchicin sensitivity antibody
      • Doxorubicin resistance antibody
      • GP170 antibody
      • MDR1 antibody
      • MDR1_HUMAN antibody
      • Multidrug resistance 1 antibody
      • Multidrug resistance protein 1 antibody
      • P glycoprotein 1 antibody
      • P gp antibody
      • P-glycoprotein 1 antibody
      • PGY1 antibody
      see all

    Anti-P Glycoprotein antibody [F4] (ab80594) 使用論文

    This product has been referenced in:
    • Chu TM  et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). WB . Read more (PubMed: 7915520) »
    • Chu TM  et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). WB, ICC/IF ; Chinese Hamster . Read more (PubMed: 7503940) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    Below is a link to our thorough western blot guide, which includes tips on sample preparation, running, and transfering large proteins.

    http://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    For yo...

    Read More



    I can confirm that it should not be necessary to prepare a membrane fraction in order to detect this protein.

    In case you are experiencing high background or to enrich the protein when low signal occurs, I would recommend preparing ...

    Read More

    Thank you for passing on that reply.

    I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

    As I mentioned in my previous email, I would be happy to provide the ...

    Read More

    Thank you for your enquiry.

    I can confirm that the epitope of ab80594 Anti-P Glycoprotein antibody [F4] is within the extracellular amino terminal end of the protein.

    I hope this will be helpful. If you have any further questions,...

    Read More

    Thank you for your enquiry and your interest in our products.

    Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

    Read More

    Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"