Anti-P cadherin 抗体 [MM0508-9V11] (ab89900)

製品の概要

  • 製品名
    Anti-P cadherin antibody [MM0508-9V11]
    P cadherin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0508-9V11] to P cadherin
  • アプリケーション
    適用あり: WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human recombinant P cadherin extracellular domain.

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute the antibody with 200µl sterile PBS and the final concentration is 0.5mg/ml.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    filter sterilised
  • ポリ/モノ
    モノクローナル
  • クローン名
    MM0508-9V11
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • 組織特異性
    Expressed in some normal epithelial tissues and in some carcinoma cell lines.
  • 関連疾患
    Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • 配列類似性
    Contains 5 cadherin domains.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Anti-P cadherin antibody [MM0508-9V11] 画像

  • Overlay histogram showing A431 cells stained with ab89900 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89900, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-P cadherin antibody [MM0508-9V11] (ab89900) 使用論文

ab89900 has not yet been referenced specifically in any publications.

Product Wall

Application
Flow Cytometry
Sample
Human Cell (Human embryonic stem cells)
Permeabilization
No
Gating Strategy
Isotype control
Specification
Human embryonic stem cells
Fixation
unfixed
Username

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Verified customer

投稿 Jun 08 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human embryonic stem cells)
Permeabilization
Yes - 0.1% Triton
Specification
Human embryonic stem cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 May 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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