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Our Abpromise guarantee covers the use of ab87312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1 - 1/3. Can be blocked with Oxytocin Receptor peptide (ab173096). Preliminary experiments in lysates of cell lines A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?|
|ELISA||Use at an assay dependent concentration. Peptide ELISA antibody detection limit dilution 1:4000|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|IHC-P||Use a concentration of 2 µg/ml.|
Immunohistochemical analysis of paraffin embedded Human prostatic cancer tissue labeling Oxytocin Receptor with ab87312 at 2 ug/ml.