Anti-Oxytocin 抗体 (ab67457)
Key features and details
- Rabbit polyclonal to Oxytocin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Oxytocin antibody -
製品の詳細
Rabbit polyclonal to Oxytocin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Human adult pituitary gland tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67457の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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細胞内局在
Secreted -
参照データベース
- Entrez Gene: 5020 Human
- Entrez Gene: 25504 Rat
- Omim: 167050 Human
- SwissProt: P01178 Human
- SwissProt: P01179 Rat
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別名
- Ocytocin antibody
- OT antibody
- OT NPI antibody
see all
画像
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Anti-Oxytocin antibody (ab67457) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) )
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Additional bands at: 10 kDa (possible cleavage fragment)
We hypothesize that the 12 and 10 kDa bands represent the pro and mature form of Oxytocin, respectively. -
ICC/IF image of ab67457 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67457 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab67457 は 3 報の論文で使用されています。
- Huang S et al. Density-induced social stress alters oxytocin and vasopressin activities in the brain of a small rodent species. Integr Zool 16:149-159 (2021). PubMed: 32652776
- Huang S et al. Population variation alters aggression-associated oxytocin and vasopressin expressions in brains of Brandt's voles in field conditions. Front Zool 18:56 (2021). PubMed: 34717666
- Kaneko Y et al. Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors. Cell Physiol Biochem 51:1982-1995 (2018). PubMed: 30513524