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Oxoguanine 8 adsorbed onto alumina.
This product was changed from ascites to tissue culture supernatant on 27th May 2016. The following lots are from ascites and are still in stock as of 27th May 2016:
Lot numbers higher than GR263316-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab64548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution. PFA fixed cells.|
Immunocytochemistry/ Immunofluorescence analysis of U2OS cells labeling Oxoguanine 8 with ab64548 at 1/500 dilution. Cells were fixed and permeabilized with methanol and blocked with 3% BSA for 1 hour at 21°C. A goat anti-mouse Alex Fluor 488 was used as the secondary antibody at 1/500 dilution.
Ab64548 detects 8-Oxoguanine in HeLa cells fixed with paraformaldehyde.