Anti-Oxoguanine 8 抗体 [2Q2311] (ab64548)


  • 製品名Anti-Oxoguanine 8 antibody [2Q2311]
    Oxoguanine 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2Q2311] to Oxoguanine 8
  • 特異性ab64548 recognizes Oxoguanine 8. In competitive ELISA, it showed no reactivity with dGMP,dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range. In typical biological conditions, the antibody only recognizes 8-oxoguanine.
  • アプリケーション適用あり: IHC-P, ELISA, ICCmore details
  • 種交差性
    Reacts with 8 Oxoguanine
  • 免疫原

    Oxoguanine 8 adsorbed onto alumina.

  • ポジティブ・コントロール
    • Nuclei of nutrient-deprived HeLa and Cos7 cells.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 27th May 2016. The following lots are from ascites and are still in stock as of 27th May 2016:

    GR263316-1, GR238689-1

    Lot numbers higher than GR263316-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.



Our Abpromise guarantee covers the use of ab64548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/400.
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution. PFA fixed cells.


  • 関連性Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1.
  • 細胞内局在Nuclear
  • 別名
    • 8 Oxoguanine antibody
    • 8 hydroxyguanine antibody
    • 8 Oxo 7,8 dihydroguanine antibody
    • 8 oxo Gua antibody
    • 8 oxoG antibody
    see all

Anti-Oxoguanine 8 antibody [2Q2311] 画像

  • ab64548 staining Oxoguanine 8 in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
    Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab64548 at 1/400 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each R
  • Immunocytochemistry/ Immunofluorescence analysis of U2OS cells labeling Oxoguanine 8 with ab64548 at 1/500 dilution. Cells were fixed and permeabilized with methanol and blocked with 3% BSA for 1 hour at 21°C. A goat anti-mouse Alex Fluor 488 was used as the secondary antibody at 1/500 dilution. 

    See Abreview

  • Ab64548 detects 8-Oxoguanine in HeLa cells fixed with paraformaldehyde.

Anti-Oxoguanine 8 antibody [2Q2311] (ab64548) 使用論文

This product has been referenced in:
  • Kilinc S  et al. Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage. Mol Cell Neurosci 74:58-70 (2016). IF ; Mouse . Read more (PubMed: 26947098) »
  • Puente BN  et al. The oxygen-rich postnatal environment induces cardiomyocyte cell-cycle arrest through DNA damage response. Cell 157:565-79 (2014). Read more (PubMed: 24766806) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2os)
Permeabilization Yes - Methanol
Specification U2os
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Methanol

Abcam user community

Verified customer

投稿 Oct 27 2016

Application Western blot
Sample Human Cell lysate - whole cell (HeLa and HCT15)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (0)
Loading amount 0.15 µg
Treatment 250uM H2O2 for 1 hour
Specification HeLa and HCT15
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Janice Ortega

Verified customer

投稿 May 04 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (heart)
Specification heart
Permeabilization Yes - 0.3%TritonX
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse lung exposed to cigarette smoke)
Specification mouse lung exposed to cigarette smoke
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate
Permeabilization Yes - Triton X-100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Dr. Jim Hsiao

Verified customer

投稿 Mar 15 2013

Thank you for contacting us. Yes, this product has been tested in mouse. Oxoguanine 8 is also the same across all species so the product has no species reactivity listed because it can react with all species. Please let me know if ou have further quest...

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Thank you for contacting us.
The antibody has not been tested in this experiment so we will not be able to guarantee it. However please provide customer details I will be happy to provide 100% Abtrial discount.
I hope this information is help...

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Thank you for contacting us.

I confirm this antibody has been tested in a competitive ELISA assay.

Plates were coated with oxo8G-casein (or 8oxoG-BSA) conjugate per well and incubated overnight at 4°C. The samples and standards ...

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Thank you for contacting us and your interest in our products.
We do not currently have a protocol to share with the anti-Anti-Oxoguanine 8 antibody [2Q2311] (ab64548) used in imaging of live cells as this is not an experiment we have tried as yet...

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Thank you for contacting us.

You can try starting dilution of 1/10,000. Please note the dilution should be determined by the end user so we recommend trying range of dilutions for optimal results.

I hope this information is helpful to...

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Thanks so much fortaking the time to send alongthis reference! Your email with the reference information will now also appear under the 'Scientific Support' tab for ab64548.

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