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Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
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Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase
|OAA Assay Buffer||WM||1 x 25ml|
|OAA Enzyme Mix||Purple||1 vial|
|OAA Probe (in DMSO)||Red||1 x 200µl|
|OAA Standard (10 µmol)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)
Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted