製品の概要

  • 製品名Oxaloacetate Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    > 2 µM
  • 検出範囲
    2 µM - 200 µM
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase.
  • 別名
    • OAA
    • Oxaloacetic acid

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Oxaloacetate Assay Kit 画像

  • Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)

  • Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted

  • Oxaloacetate standard curve generated following the kit protocol.

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Oxaloacetate Assay Kit (ab83428) 使用論文

This product has been referenced in:
  • Long LH & Halliwell B The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism. Biochem Biophys Res Commun 417:446-50 (2012). Functional Studies . Read more (PubMed: 22166196) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"