製品の概要

  • 製品名Anti-OVOL2 antibody
    OVOL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OVOL2
  • アプリケーション適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 226-275 (QEDLYLHVNS AHPGSSFLKK TSKKLAALLQ GKLTSAHQEN TSLSEEEERK) of human OVOL2 (NP_067043).

  • ポジティブ・コントロール
    • 721_B cell lysate; human cornea

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:62500.

IHC-Fr 1/250.

ターゲット情報

  • 機能DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.
  • 組織特異性Expressed in testis, ovary, heart and skeletal muscle.
  • 配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA504H3.3 antibody
    • EUROIMAGE566589 antibody
    • hOvo 2 antibody
    • hOvo2 antibody
    • Ovo like 2 (Drosophila) antibody
    • Ovo like 2 antibody
    • ovo-like zinc finger 2 antibody
    • OVOL 2 antibody
    • OVOL2 antibody
    • OVOL2_HUMAN antibody
    • Transcription factor Ovo like 2 antibody
    • Transcription factor Ovo-like 2 antibody
    • Zinc finger protein 339 antibody
    • ZNF 339 antibody
    • ZNF339 antibody
    see all

Anti-OVOL2 antibody 画像

  • Anti-OVOL2 antibody (ab83265) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Gel concentration: 12%
  • Immunofluorescent staining of OVOL2 in human corneal endothelium with ab83265 at a dilution of 1/250. a: signal for Propidium iodide and OVOL2 b: signal for OVOL2 only c: corneal epithelium.

    Corneal epithelium as well as limbal epithelium was positive in the cytoplasm. Stromal keratocytes and endothelium was positive predominantly in the cell nucleus

Anti-OVOL2 antibody (ab83265) 使用論文

ab83265 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83265.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"