製品の概要

  • 製品名
    Anti-Ovalbumin antibody [6C8]
    Ovalbumin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6C8] to Ovalbumin
  • 由来種
    Mouse
  • 特異性
    The antibody reacts strongly with Ovalbumin (OA) in denatured and modified forms (native OA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA, with OA directly coated onto the microtiterwell. In dotblotting it reacts with both native and denatured OA adsorbed onto nitrocellulose. In Western blotting after SDS-PAGE, the antibody reacts with OA in reduced as well as unreduced forms.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Chicken
  • 免疫原

    Full length native protein (purified) corresponding to Chicken Ovalbumin. Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35°C and then autoclaved for 1 h at 110°C.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項（精製）
    Immunized i.p. with immunogen adsorbed onto Al(OH)3.
  • ポリ/モノ
    モノクローナル
  • クローン名
    6C8
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/4000.
WB 1/1000. Detects a band of approximately 45 kDa.

ターゲット情報

  • 機能
    Storage protein of egg white. Lack protease inhibitory activity.
  • 組織特異性
    Major protein of egg white.
  • 配列類似性
    Belongs to the serpin family. Ov-serpin subfamily.
  • 翻訳後修飾
    The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Allergen Gal d II antibody
      • Egg albumin antibody
      • OVAL_CHICK antibody
      • Ovalbumin antibody
      • Plakalbumin antibody
      • Serpin peptidase inhibitor antibody
      • SERPINB14 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Grødeland G  et al. The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype. PLoS One 8:e80008 (2013). WB ; Human . Read more (PubMed: 24244595) »
    • Ding Q  et al. RAFTsomes containing epitope-MHC-II complexes mediated CD4+ T cell activation and antigen-specific immune responses. Pharm Res 30:60-9 (2013). Read more (PubMed: 22878683) »

    See all 5 Publications for this product

    ab17293 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    10 reviews or Q&A

    10
    Votes: Highest First

    What was the rationale for preparing the immunogen by heating at 105C for one hour? Is ovalbumin difficult to denature? Has the epitope been mapped?

    The two articles at the PubMed addresses below discuss the rationale for the preparation of the immunogen that generated the monoclonal antibody ab17293. The epitope that it detects (LEPINF, aa130-135) is listed in Table 1 of the 2014 paper, which refe...

    Read More

    Dear All,
    a customer of ours would like to know which are the lots available for code ab17293.

    Thanks and kind regards

    Thank you for contacting us.

    There is just one lot available for ab17293. The lot number is: 1247369.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    thank you for your e-mail I spoke to my customer and finally she prefers to get a a credit note.

    Thanks again and
    Kind regards

    Thank you for your note.

    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab17293. For your information, the internal reference note for this cred...

    Read More

    I have just received a feedback form the customer. She has tested the Ab yout sent as replacement but even using it at different concentration it did not work yet.
    The customer would like to receive anew vial but from a different lot.
    Can you p...

    Read More

    Thank you for your message and for your updates.

    I am very sorry about this news. Currently, we have the same batch in stock and we do not have new batch available for a while. I can offer a credit note which can be used for any products which...

    Read More

    Our customer would accept another vial of the same lot, but if this vial does not work again, she wants to receive another free vial when a new lot will be available.
    Please let me know if you can accept this condition.

    Kind regards

    Thank you for your response.

    This is to let you know that I have placed a new order for you - for one vial ofab17293 as a free of charge replacement and the new order number is 1083493.

    I hope the second vial will work as it is expe...

    Read More

    Our customer would like to know when a new batch will be available.
    If the new batch will be soon available, our customer prefers to wait for it.

    Please let me know

    Kind regards

    Thank you for your response.

    We have not had any complaint about this antibody since 2008. According to our record, we have sold several vials of this batch without any problem. This implies that it is very unlikely that the problem is batch-...

    Read More

    Thank you for your reply. Unfortunately they haven't the old vial anymore so they are not able to provide us with the batch number. I'm sorry for that!

    Kind regards

    Thank you for your response.

    I can offer you a new vial as free of charge replacement or a credit note which can be used in future purchase. The product will be from the same purification since currently we do not have a new batch.

    ...

    Read More

    Dear All,
    please find in attchment two complaint forms for the same Ab ab17293 (IHC and ELISA forms), filled by our customer.
    I did not submit it online because the customer is the same so that I thought it was better to send them in the same...

    Read More

    Thank you for your enquiry regarding ab17293 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    I would like to reassure you that our Abpromise a...

    Read More

    I want to ask you some information about the primary antibody mouse
    monoclonal to ovalbumin (ab17293) because I bought it about a year ago and I
    stored it as it is written in the technical support such as at 4C but it
    doesn't work anymor...

    Read More

    Thank you for your inquiry.


    We do recommend that you store ab17293 at +4C in the dark. Generally, antibodies stored at +4C are viable for up to a year. I wouldn't recommend freezing it. Since we haven't tested the antibody after it's be...

    Read More

    Good morning, I have a technical question about your monoclonal antibody to ovalbumin (ab17293). Can you tell me if the epitope of ovalbumin (recognized by this antibody) is repeated in this protein? Is it possible for this antibody bind different ...

    Read More

    Thank you for your inquiry. The immunogen for this antibody was the full length native and purified protein (chicken). The lab confirmed that the epitope that the antibody recognizes was not mapped. therefore I am unable to answer your ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録