製品の概要
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製品名Anti-Ovalbumin antibody [6C8]
Ovalbumin 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [6C8] to Ovalbumin
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特異性The antibody reacts strongly with Ovalbumin (OA) in denatured and modified forms (native OA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA, with OA directly coated onto the microtiterwell. In dotblotting it reacts with both native and denatured OA adsorbed onto nitrocellulose. In Western blotting after SDS-PAGE, the antibody reacts with OA in reduced as well as unreduced forms.
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アプリケーション適用あり: ELISA, WBmore details
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種交差性交差種: Chicken
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免疫原
Full length native protein (purified) corresponding to Chicken Ovalbumin. Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35°C and then autoclaved for 1 h at 110°C.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 15mM Sodium Azide
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4 -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）Immunized i.p. with immunogen adsorbed onto Al(OH)3.
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ポリ/モノモノクローナル
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クローン名6C8
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アイソタイプIgG1
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab17293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/4000. | |
| WB | 1/1000. Detects a band of approximately 45 kDa. |
ターゲット情報
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機能Storage protein of egg white. Lack protease inhibitory activity.
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組織特異性Major protein of egg white.
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配列類似性Belongs to the serpin family. Ov-serpin subfamily.
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翻訳後修飾The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 396058 Chicken
- SwissProt: P01012 Chicken
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別名
- Allergen Gal d II antibody
- Egg albumin antibody
- OVAL_CHICK antibody
see all
プロトコール
Anti-Ovalbumin antibody [6C8] (ab17293) 使用論文
This product has been referenced in:
- Grødeland G et al. The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype. PLoS One 8:e80008 (2013). WB ; Human . Read more (PubMed: 24244595) »
- Ding Q et al. RAFTsomes containing epitope-MHC-II complexes mediated CD4+ T cell activation and antigen-specific immune responses. Pharm Res 30:60-9 (2013). Read more (PubMed: 22878683) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"