Anti-Otx2 抗体 (ab76748)
Key features and details
- Rabbit polyclonal to Otx2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
-
製品名
Anti-Otx2 antibody
Otx2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Otx2 -
由来種
Rabbit -
特異性
Reacts specifically with 31 kDa Otx2 protein. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Rat -
免疫原
Synthetic peptide derived from the N terminal domain of human Otx2
-
ポジティブ・コントロール
- This antibody gave a positive siganl in OTX2 Overexpression Lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
-
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76748の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
|
特記事項 |
---|
WB
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). |
ターゲット情報
-
機能
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. -
組織特異性
Expressed in brain. -
関連疾患
Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures. -
配列類似性
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
発生段階
Embryo. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 305858 Rat
- SwissProt: Q64201 Rat
- Unigene: 35222 Rat
-
別名
- CPHD6 antibody
- Homeobox protein OTX2 antibody
- MCOPS 5 antibody
see all
画像
-
All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml
Lane 1 : OTX1 Recombinant Protein
at 0.1 µg
Lane 2 : OTX2 Overexpression Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab76748 は 2 報の論文で使用されています。
- Moreno-Mármol T et al. Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis. Elife 10:N/A (2021). PubMed: 34545806
- Japp AS et al. High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors. J Neuropathol Exp Neurol 74:110-20 (2015). PubMed: 25575132