製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to OTUD4
  • アプリケーション
    適用あり: ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 18 aa from near the C-terminus of Human OTUD4 (NP_001096123).

  • ポジティブ・コントロール
    • Daudi cell lysate, Daudi cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 124 kDa.Can be blocked with OTUD4 peptide (ab217680).
ICC Use a concentration of 2.5 µg/ml.

ターゲット情報

Anti-OTUD4 antibody 画像

  • Lane 1 : Anti-OTUD4 antibody (ab106368) at 0.25 µg/ml
    Lane 2 : Anti-OTUD4 antibody (ab106368) at 0.5 µg/ml

    Lane 1 : Daudi (human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate


    Predicted band size : 124 kDa
  • ab106368, at 2.5 ug/ml, staining OTUD4 in Daudi cells by immunocytochemistry.
  • Immunofluorescence of OTUD4 in Daudi cells using ab106368 at 20 ug/ml.

Anti-OTUD4 antibody (ab106368) 使用論文

This product has been referenced in:
  • Esquerdo M  et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . Read more (PubMed: 26934049) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (lymphoid)
Specification
lymphoid
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録