The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Widely expressed. Expressed at higher level in brain.
Belongs to the peptidase C65 family. Contains 1 OTU domain.
All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution
Lane 1 : Extract from HepG2 cells Lane 2 : Extract from Jurkat cells Lane 3 : Extract from COLO 205 cells Lane 4 : Extract from HT-29 cells Lane 5 : Extract from 293 cells Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa Observed band size: 27 kDa Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.