製品の概要

  • 製品名Anti-OTUB1 antibody
    OTUB1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to OTUB1
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide: C-

    YKEYAEDDNIYQQK

    corresponding to internal sequence amino acids 58-71 of human OTUB1 (NP_060140.2).

  • ポジティブ・コントロール
    • Human brain (cerebellum) and, mouse and rat brain lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab82154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.
    • 組織特異性Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.
    • 配列類似性Belongs to the peptidase C65 family.
      Contains 1 OTU domain.
    • ドメインIn addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Deubiquitinating enzyme OTUB1 antibody
      • hOTU1 antibody
      • HSPC263 antibody
      • OTB1 antibody
      • OTU domain containing ubiquitin aldehyde binding protein 1 antibody
      • OTU domain, ubiquitin aldehyde binding 1 antibody
      • OTU domain-containing Ubal-binding protein 1 antibody
      • OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody
      • OTU-domain Ubal-binding 1 antibody
      • OTU1 antibody
      • Otub1 antibody
      • OTUB1_HUMAN antibody
      • Otubain 1 antibody
      • Otubain-1 antibody
      • Ubiquitin specific processing protease OTUB1 antibody
      • Ubiquitin thioesterase OTUB1 antibody
      • ubiquitin-specific protease otubain 1 antibody
      • Ubiquitin-specific-processing protease OTUB1 antibody
      see all

    Anti-OTUB1 antibody 画像


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 31 kDa


      Exposure time : 1 minute

      Image courtesy of an Anonymous review

      Anti-OTUB1 antibody (ab82154) reactivity with reduced HEK293 cell lysate after transient transfection (48h) of control siRNA (lane 1-3) or siRNA targeting OTUB1 (lane 4 and 5). After SDS-PAGE, membranes were blocked in 5% milk TBS + 0.1% Tween for 1h at 25°C before incubation with ab82154 (1:1000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Goat HRP-conjugated secondary antibody before developing with ECL. Anti-tubulin blot included as a loading control. 

      See Abreview

    • Anti-OTUB1 antibody (ab82154) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 31 kDa
      Observed band size : 31 kDa


      Exposure time : 1 hour

    Anti-OTUB1 antibody (ab82154) 使用論文

    ab82154 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample Human Cell (HaCaT)
    Specification HaCaT
    Permeabilization Yes - Triton X-100 plus
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 14 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Mouse Cell lysate - whole cell (NMuMG cells)
    Specification NMuMG cells
    Treatment control or OTUB1 siRNA (300pM per 10 cm dish, 48h)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Ms. Lina Herhaus

    Verified customer

    投稿 Oct 11 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Treatment control or OTUB1 siRNA (300pM per 10 cm dish, 48h)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"