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Our Abpromise guarantee covers the use of ab9986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent dilution. PubMed: 20513175|
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 46 kDa).Can be blocked with Recombinant Osteoprotegerin protein (ab110188).|
|ELISA||Use a concentration of 0.5 µg/ml. Allows the detection of 0.2-0.4 ng/well or recombinant hOPG.|
ab9986 staining Osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with PBS + 1% BSA + 5% NGS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. ab7090, a HRP-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.