製品の概要

  • 製品名Anti-Osteopontin antibody
    Osteopontin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Osteopontin
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    DPKSKEEDKHLK

    , corresponding to C terminal amino acids 288-299 of Human Osteopontin

  • ポジティブ・コントロール
    • Human thymus, tonsil and spleen lysate. Jurkat and MOLT4 lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab36125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

Blocking peptide available ab45753.

ターゲット情報

  • 機能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 組織特異性Bone. Found in plasma.
  • 配列類似性Belongs to the osteopontin family.
  • 翻訳後修飾Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody 画像

  • Anti-Osteopontin antibody (ab36125) at 0.3 µg/ml + Human Thymus lysate (RIPA buffer, 30µg total protein per lane).

    Predicted band size : 34 kDa
  • Anti-Osteopontin antibody (ab36125) at 1 µg/ml + lysate prepared from MOLT4 cells (RIPA buffer) at 30 µg

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Ab36125 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

    A) Staining in the germinal centre and interfollicular region.

    B) Positive cells in intraepithelial region.

Anti-Osteopontin antibody (ab36125) 使用論文

This product has been referenced in:
  • Wang Y  et al. Peptide nanofibers preconditioned with stem cell secretome are renoprotective. J Am Soc Nephrol 22:704-17 (2011). WB ; Mouse . Read more (PubMed: 21415151) »
  • Chen KC  et al. Evaluation of subchronic toxicity of pet food contaminated with melamine and cyanuric acid in rats. Toxicol Pathol 37:959-68 (2009). Read more (PubMed: 19801503) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab36125.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"