The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.
Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.
Use a concentration of 0.5 - 1 µg/ml.
Use at an assay dependent dilution. PubMed: 17224478
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Bone. Found in plasma.
Belongs to the osteopontin family.
Extensively phosphorylated on clustered serine residues. N- and O-glycosylated. Phosphorylation sites are present in the extracelllular medium.
Sharon Y et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res75:963-73 (2015).
Read more (PubMed: 25600648) »
Shibasaki M et al. Pitavastatin Reduces Inflammation in Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice with Late Stage Renal Disease. PLoS One10:e0138047 (2015).
Read more (PubMed: 26367531) »