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Recombinant human Osteopontin
Our Abpromise guarantee covers the use of ab69498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20688384|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 33 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 µg/ml.|
ab69498 staining Osteopontin in Human cancellous bone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.25% Casein for 10 minutes at 23°C. Samples were incubated with primary antibody (1/50 in 10% human serum in TBS) for 1 hour at 23°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG2a polyclonal (1/500) was used as the secondary antibody.