The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 29 kDa.
Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi
encoded on its Plasmid lp54, is a major component of the spirochete's
extracellular matrix. OspA probably serves as a lipid-anchor. The
spirochetes migrate from the tick midgut during feeding to its salivary
glands and are thus transmitted to the mammal host. This transition may
be facilitated by changes in expression of some B. burgdorferi genes.
Upon transmission of the spirochete from the Ixodes tick to mammalian
host, the transcript level of OspA can change. It is believed that
expression of the various proteins associated with the spirochete may be
regulated by the changes in tick life cycle, changes in conditions during
tick feeding (such as temperature, pH, and nutrients) and/or in
coordination with the course of infection of the mammal host. B.
burgdorferi can attach to (and also differentially express antigens in)
diverse tissues within the vertebrate host and the tick vector, suggesting
that physiological factors other than pH and temperature may play roles in
modulating B. burgdorferi gene expression.
Cell outer membrane; Lipid-anchor.
outer surfce protein A antibody
Western blot - Anti-ospA antibody (ab106081)
All lanes : Anti-ospA antibody (ab106081) at 1/1000 dilution
Lane 1 : MBP-ospA fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary Goat-anti-Rabbit HRP at 1/40000 dilution