Rabbit polyclonal to OSCAR
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human OSCAR.
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Bone Tumor; Spleen.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
Belongs to the leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family.
Contains 2 Ig-like (immunoglobulin-like) domains.
Secreted and Cell membrane.
Information by UniProt
- hOSCAR antibody
- hOSCARM2 antibody
- hOSCARM3 antibody
Western blot - OSCAR antibody (ab93817)
All lanes :
Anti-OSCAR antibody (ab93817) at 1 µg/mlLane 1 :
Bone Marrow (Human) Tissue LysateLane 2 :
Bone Tumor (Human) Tissue LysateLane 3 :
Human spleen tissue lysate - total protein (ab29699
Lysates/proteins at 10 µg per lane.Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
30 kDaObserved band size :
28 kDa (why is the actual band size different from the predicted?
)Additional bands at :
200 kDa,56 kDa,65 kDa. We are unsure as to the identity of these extra bands.Exposure time :
has not yet been referenced specifically in any publications.
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