製品の概要

  • 製品名Anti-OS9 antibody [EPR4272(2)]
    OS9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4272(2)] to OS9
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human OS9.

  • ポジティブ・コントロール
    • NIH3T3, 293T, HL-60, and HeLa cell lysates, Mouse testis tissue
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
    • 組織特異性Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
    • 配列類似性Belongs to the OS-9 family.
      Contains 1 PRKCSH domain.
    • 翻訳後修飾Intramolecular disulfide bonds.
      Isoform 1 and isoform 2 are N-glycosylated.
    • 細胞内局在Endoplasmic reticulum lumen.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Amplified in osteosarcoma 9 antibody
      • amplified in osteosarcoma antibody
      • ERLEC2 antibody
      • OS-9 antibody
      • Os9 antibody
      • OS9_HUMAN antibody
      • Osteosarcoma amplified 9 endoplasmic reticulum lectin antibody
      • Protein OS-9 antibody
      see all

    Anti-OS9 antibody [EPR4272(2)] 画像

    • All lanes : Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HL-60 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 76 kDa
    • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.

    Anti-OS9 antibody [EPR4272(2)] (ab109510) 使用論文

    This product has been referenced in:
    • Sun S  et al. Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. Proc Natl Acad Sci U S A 111:E582-91 (2014). Read more (PubMed: 24453213) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8% Bis-Tris gel, MOPS buffer)
    Sample Human Cell lysate - whole cell (HEK293T)
    Specification HEK293T
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    投稿 Mar 02 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"