製品の概要
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製品名Anti-OS9 antibody
OS9 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to OS9
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由来種Rabbit
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アプリケーション適用あり: WB, IP, ICC/IFmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human OS9.
(Peptide available as ab23317.)
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ポジティブ･コントロール
- WB: HeLa, HEK293 and NIH3T3 whole cell lysates and mouse heart, spleen, and liver tissue lysates. ICC/IF: HeLa cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab19853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). | |
| IP | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
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組織特異性Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
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配列類似性Belongs to the OS-9 family.
Contains 1 PRKCSH domain. -
翻訳後修飾Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated. -
細胞内局在Endoplasmic reticulum lumen.
- Information by UniProt
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参照データベース
- Entrez Gene: 10956 Human
- Entrez Gene: 216440 Mouse
- Entrez Gene: 362891 Rat
- Omim: 609677 Human
- SwissProt: Q13438 Human
- SwissProt: Q8K2C7 Mouse
- SwissProt: Q5RKH6 Rat
- Unigene: 527861 Human
see all -
別名
- Amplified in osteosarcoma 9 antibody
- amplified in osteosarcoma antibody
- ERLEC2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (ab19853)IHC image of OS9 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19853, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-OS9 antibody (ab19853)All lanes : Anti-OS9 antibody (ab19853) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 30 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-OS9 antibody (ab19853)ICC/IF image of ab19853 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19853, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Kizhakkedath P et al. Degradation routes of trafficking-defective VLDLR mutants associated with Dysequilibrium syndrome. Sci Rep 8:1583 (2018). Read more (PubMed: 29371607) »
- Erzurumlu Y & Ballar P Androgen Mediated Regulation of Endoplasmic Reticulum-Associated Degradation and its Effects on Prostate Cancer. Sci Rep 7:40719 (2017). Read more (PubMed: 28091582) »
レビューと Q&A
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