Anti-ORP8 抗体 [mAbcam 60110] (ab60110)

製品の概要

  • 製品名Anti-ORP8 antibody [mAbcam 60110]
    ORP8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 60110] to ORP8
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Horse, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ORP8.

    (Peptide available as ab75267.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human kidney tissue lysate and in the following whole cell lysates: HEK293; THP1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性ORP8 is a member of the oxysterol-binding protein related protein family. In humans, the gene family consists of 12 members, and extensive splice variation increases the number of encoded protein products substantially. The ORPs have been implicated as sterol sensors that regulate a number of cellular functions including sterol and neutral lipid metabolism, intracellular lipid transport, membrane trafficking and cell signaling. ORP8 has been recently shown to act as a sterol sensor that affects the reverse cholesterol transport process via modulation of ABCA1 expression and macrophage cholesterol efflux.
  • 細胞内局在Endoplasmic reticulum
  • 参照データベース
  • 別名
    • DKFZp686A11164 antibody
    • KIAA1451 antibody
    • MGC126578 antibody
    • MGC133203 antibody
    • MST120 antibody
    • MSTP120 antibody
    • ORP 8 antibody
    • OSBP related protein 8 antibody
    • OSBP10 antibody
    • OSBPL8 antibody
    • Oxysterol binding protein like 8 antibody
    • Oxysterol binding protein like protein 8 antibody
    • Oxysterol binding protein related protein 8 antibody
    see all

Anti-ORP8 antibody [mAbcam 60110] 画像

  • All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 101 kDa
    Observed band size : 101 kDa


    Exposure time : 8 minutes

    We recommend using 3% milk as the blocking agent in Western Blot.

  • All lanes : Anti-ORP8 antibody [mAbcam 60110] (ab60110) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 101 kDa
    Observed band size : 101 kDa
    Additional bands at : 44 kDa,55 kDa,80 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    1% BSA was used as a blocking agent.

  • Overlay histogram showing HEK293 cells stained with ab60110 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60110, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ORP8 antibody [mAbcam 60110] (ab60110) 使用論文

ab60110 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa, Mel202, A431)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification HeLa, Mel202, A431
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jul 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"