製品の概要

  • 製品名Anti-ORP8 antibody
    ORP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ORP8
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 755-804 (

    YSSPEPDIQDSSGSEAQSVKPSTRRKKGIELGDIQSSIESIKQTQEEIKR

    ) of Human ORP8 (NP_001003712).

  • ポジティブ・コントロール
    • OVCAR3 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性ORP8 is a member of the oxysterol-binding protein related protein family. In humans, the gene family consists of 12 members, and extensive splice variation increases the number of encoded protein products substantially. The ORPs have been implicated as sterol sensors that regulate a number of cellular functions including sterol and neutral lipid metabolism, intracellular lipid transport, membrane trafficking and cell signaling. ORP8 has been recently shown to act as a sterol sensor that affects the reverse cholesterol transport process via modulation of ABCA1 expression and macrophage cholesterol efflux.
  • 細胞内局在Endoplasmic reticulum
  • 参照データベース
  • 別名
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Anti-ORP8 antibody 画像

  • IHC image of ORP8 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99069, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ORP8 antibody (ab99069) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Predicted band size : 101 kDa
    Gel concentration: 6-18%

Anti-ORP8 antibody (ab99069) 使用論文

ab99069 has not yet been referenced specifically in any publications.

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