製品の概要

  • 製品名Anti-ORP150 antibody [EPR5890]
    ORP150 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5890] to ORP150
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • Human fetal liver, Rat liver, Rat pancreas, 293T, MCF7, MDA-MB-435S, SH-SY5Y and HeLa lysates; Human liver tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 111 kDa).Can be blocked with ORP150 peptide (ab156891).
IHC-P 1/250 - 1/500.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
    • 組織特異性Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
    • 配列類似性Belongs to the heat shock protein 70 family.
    • 細胞内局在Endoplasmic reticulum lumen.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 150 kDa oxygen regulated protein antibody
      • 150 kDa oxygen-regulated protein antibody
      • 170 kDa glucose regulated protein antibody
      • 170 kDa glucose-regulated protein antibody
      • DKFZp686N08236 antibody
      • FLJ94899 antibody
      • FLJ97572 antibody
      • glucose regulated protein 170 antibody
      • Grp 170 antibody
      • GRP-170 antibody
      • Grp170 antibody
      • HSP 12A antibody
      • HSP12A antibody
      • Hyou1 antibody
      • HYOU1_HUMAN antibody
      • Hypoxia up regulated 1 antibody
      • hypoxia up-regulated 1 precursor antibody
      • Hypoxia up-regulated protein 1 antibody
      • Hypoxia upregulated 1 antibody
      • Orp 150 antibody
      • ORP-150 antibody
      • Orp150 antibody
      • oxygen regulated protein (150kD) antibody
      see all

    Anti-ORP150 antibody [EPR5890] 画像

    • Overlay histogram showing HepG2 cells stained with ab124884 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124884, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ORP150 antibody [EPR5890] (ab124884) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Rat liver lysate
      Lane 3 : Rat pancreas lysate
      Lane 4 : 293T lysate
      Lane 5 : MCF7 lysate
      Lane 6 : MDA-MB-435S lysate
      Lane 7 : SH-SY5Y lysate
      Lane 8 : HeLa lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 111 kDa
    • ab124884, at 1/250 dilution, staining ORP150 in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-ORP150 antibody [EPR5890] (ab124884) 使用論文

    This product has been referenced in:
    • Chiu HC  et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . Read more (PubMed: 24671231) »

    See 1 Publication for this product

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