Anti-ORP150 抗体 - Carboxyterminal end (ab56051)

製品の概要

  • 製品名Anti-ORP150 antibody - Carboxyterminal end
    ORP150 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ORP150 - Carboxyterminal end
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to C-terminal residues of rat ORP150

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab56051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 111 kDa.Can be blocked with Rat ORP150 peptide (ab89014). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
  • 組織特異性Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
  • 配列類似性Belongs to the heat shock protein 70 family.
  • 細胞内局在Endoplasmic reticulum lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 150 kDa oxygen regulated protein antibody
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    • 170 kDa glucose-regulated protein antibody
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    • FLJ94899 antibody
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    • glucose regulated protein 170 antibody
    • Grp 170 antibody
    • GRP-170 antibody
    • Grp170 antibody
    • HSP 12A antibody
    • HSP12A antibody
    • Hyou1 antibody
    • HYOU1_HUMAN antibody
    • Hypoxia up regulated 1 antibody
    • hypoxia up-regulated 1 precursor antibody
    • Hypoxia up-regulated protein 1 antibody
    • Hypoxia upregulated 1 antibody
    • Orp 150 antibody
    • ORP-150 antibody
    • Orp150 antibody
    • oxygen regulated protein (150kD) antibody
    see all

Anti-ORP150 antibody - Carboxyterminal end 画像

  • IHC image of ab56051 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ORP150 antibody - Carboxyterminal end (ab56051) 使用論文

ab56051 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"