製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ORP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide: YWDRNYFNLPDIY, corresponding to C terminal amino acids 938-950 of Human ORP1.

  • ポジティブ・コントロール
    • Human muscle lysate.
  • 特記事項


    Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考
    Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ORP1 peptide (ab23188).

ターゲット情報

プロトコール

参考文献

ab10151 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Mouse and rat brain lysate, as well as cell lysate)
Loading amount
100 µg
Specification
Mouse and rat brain lysate, as well as cell lysate
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 May 01 2008

I'm sorry to hear you are having a problem with ab10151. Although I do not have the information for the detailed protocol that you followed I would like to suggest the following modifications that may improve your result: I would block in 5% milk in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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