Anti-Ornithine Carbamoyltransferase 抗体 (ab55914)
製品の概要
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製品名Anti-Ornithine Carbamoyltransferase antibody
Ornithine Carbamoyltransferase 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Ornithine Carbamoyltransferase
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
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免疫原
A region within synthetic peptide: LKGRDLLTLK NFTGEEIKYM LWLSADLKFR IKQKGEYLPL LQGKSLGMIF, corresponding to N terminal amino acids 37-86 of Human Ornithine Carbamoyltransferase
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ポジティブ･コントロール
- HepG2 cell lysate Human intestine: epithelial cells of intestinal villus
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab55914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
ターゲット情報
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組織特異性Mainly expressed in liver and intestinal mucosa.
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パスウェイNitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
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関連疾患Ornithine carbamoyltransferase deficiency
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配列類似性Belongs to the ATCase/OTCase family.
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翻訳後修飾Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
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細胞内局在Mitochondrion matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 280887 Cow
- Entrez Gene: 480878 Dog
- Entrez Gene: 5009 Human
- Entrez Gene: 18416 Mouse
- Entrez Gene: 397438 Pig
- Entrez Gene: 25611 Rat
- Entrez Gene: 101102207 Sheep
- Omim: 300461 Human
see all -
別名
- EC 2.1.3.3 antibody
- MGC129967 antibody
- MGC129968 antibody
see all
Anti-Ornithine Carbamoyltransferase antibody 画像
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Western blot - Ornithine Carbamoyltransferase antibody (ab55914)Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
Gel concentration: 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ornithine Carbamoyltransferase antibody(ab55914)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Intestine tissue labelling Ornithine Carbamoyltransferase with ab55914 at 8µg/ml. Positively stained epithelial cells of intestinal villus are indicated with arrows. Magnification: 400X
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Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab55914)All lanes : Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 1.25 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size : 40 kDa
Anti-Ornithine Carbamoyltransferase antibody (ab55914) 使用論文
This product has been referenced in:
- van Straten G et al. Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts. PLoS One 9:e100077 (2014). IHC-P ; Dog . Read more (PubMed: 24945279) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"