Anti-Ornithine Carbamoyltransferase 抗体 (ab55914)

Abreviews (1)Q&A (3)Specific References (1)

製品の概要

  • 製品名
    Anti-Ornithine Carbamoyltransferase antibody
    Ornithine Carbamoyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ornithine Carbamoyltransferase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: LKGRDLLTLK NFTGEEIKYM LWLSADLKFR IKQKGEYLPL LQGKSLGMIF, corresponding to N terminal amino acids 37-86 of Human Ornithine Carbamoyltransferase

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • HepG2 cell lysate Human intestine: epithelial cells of intestinal villus

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

画像

  • Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab55914)
    Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab55914)
    Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase antibody (ab55914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase antibody (ab55914)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Intestine tissue labelling Ornithine Carbamoyltransferase with ab55914 at 8µg/ml. Positively stained epithelial cells of intestinal villus are indicated with arrows. Magnification: 400X

  • Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab55914)
    Western blot - Anti-Ornithine Carbamoyltransferase antibody (ab55914)
    All lanes : Anti-Ornithine Carbamoyltransferase antibody (ab55914) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 40 kDa

参考文献

This product has been referenced in:
  • van Straten G  et al. Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts. PLoS One 9:e100077 (2014). IHC-P ; Dog . Read more (PubMed: 24945279) »

See 1 Publication for this product

ab55914 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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4 reviews or Q&A

10
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Inquiry: Our customer is willing to test ab55914 in ICC (intracellular staining) with the recombinant fragment of OCT (human). Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and ...

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Thank you for your enquiry.

I am very pleased to hear the customer would like to make use of our testing discount offer and test ab55914 in ICC. I can confirm this antibody has not been previously tested (and guaranteed) in this application....

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I hope this finds you well.

Do you have some update in regards to the tests discussed below?

Thank you in advance.

Have a wonderful day.

Thank you for contacting us.
I am sorry but I have as yet not recieved an update as to the restest of the antibody using a different sample (not HepG2). It may be that the tests have not been performed just yet but I will get in contact with the l...

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Product code: 55914 Inquiry: Our customer wishes to test ab55914 in ICC/IF with human samples. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website...

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Thank you for contacting us. That is indeed information that we would be interested in receiving. The antibody ab55914 has as yet not been tested in ICC/IF and if your customer would be willing to submit an Abreview for this that would be great. I h...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ornithine Carbamoyltransferase antibody

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Cell (liver)
Specification
liver
Fixative
Formalin
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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投稿 Dec 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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