製品の概要

  • 製品名
    Anti-ORMDL3 antibody
    ORMDL3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ORMDL3
  • 由来種
    Rabbit
  • 特異性
    ab107639 may cross-react with ORMDL1 and ORMDL2.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ORMDL3 aa 53-81 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N138

  • ポジティブ･コントロール
    • WB: Mouse testis tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 17 kDa.

ターゲット情報

  • 機能
    Negative regulator of sphingolipid synthesis. May indirectly regulate endoplasmic reticulum-mediated Ca(+2) signaling.
  • 組織特異性
    Widely expressed. Expressed in adult and fetal heart, brain, lung, liver, skeletal muscle and kidney. Expressed in adult pancreas and placenta and in fetal spleen abd thymus.
  • 関連疾患
    Genetic variations in ORMDL3 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • 配列類似性
    Belongs to the ORM family.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ORM1 (S. cerevisiae)-like 3 antibody
      • ORM1-like 3 (S. cerevisiae) antibody
      • ORM1-like 3 antibody
      • ORM1-like protein 3 antibody
      • ORMDL sphingolipid biosynthesis regulator 3 antibody
      • Ormdl3 antibody
      • ORML3_HUMAN antibody
      • OTTHUMP00000164334 antibody
      see all

    画像

    • Western blot - Anti-ORMDL3 antibody (ab107639)
      Western blot - Anti-ORMDL3 antibody (ab107639)
      All lanes : Anti-ORMDL3 antibody (ab107639) at 1/1000 dilution

      Lane 1 : Jurkat whole cell lysate
      Lane 2 : Mouse testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

      Predicted band size: 17 kDa



      Blocking buffer 5% NFDM/TBST
      Diluting buffer  5% NFDM/TBST

    プロトコール

    参考文献

    This product has been referenced in:
    • Yu F  et al. ORMDL3 is associated with airway remodeling in asthma via the ERK/MMP-9 pathway. Mol Med Rep 15:2969-2976 (2017). WB, IHC-P ; Mouse . Read more (PubMed: 28358425) »
    • Zhuang LL  et al. Promoter characterization and role of cAMP/PKA/CREB in the basal transcription of the mouse ORMDL3 gene. PLoS One 8:e60630 (2013). WB ; Mouse . Read more (PubMed: 23577138) »

    See all 2 Publications for this product

    ab107639 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

    10
    Votes: Highest First

    Can product Anti-ORMDL3 antibody (ab107639) detect the endogenous ORMDL3 protein in lysate samples, without overexpression?

    Can Abcam provide data that shows detection of endogenous ORMDL3 in cell lysates?

    Yes, product Anti-ORMDL3 antibody (ab107639) is able to detect endogenous ORMDL3 protein in lysate samples, without overexpression.

    Western Blot data showing use of ab107639 for the detection of endogenous ORMDL3 in Jurkat whole cell lysates a...

    Read More

    Do you carry a product that is able to detect all proteins of the ORMDL family (ORMDL1/ORMDL2/ORMDL3)?

    Is this product able to detect endogenous ORMDL1-3 in cell lysate samples, by WB?

    Yes - product Anti-ORMDL1 + ORMDL2 + ORMDL3 antibody (ab128660) is a pan-specific ORMDL family antibody. It will detect ORMDL1, ORMDL2 and ORMDL3.

    Ab128660 is able to detect endogenous ORMDL1-3 in human cell lysates by WB; reactivity against ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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