製品の概要

  • 製品名Anti-Orexin Receptor 2 antibody
    Orexin Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Orexin Receptor 2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Pig
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 263/282 of Mouse Orexin Receptor 2. Target sequence used to make the antibody:

    VQRKWKQQQPVSQPRGSGQQS

    .

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 51 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides (PubMed:9491897, PubMed:26950369). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed:9491897, PubMed:26950369).
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • ドメインThe N-terminal region is required for orexin signaling.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hcr tr2 antibody
    • Hcrt receptor 2 antibody
    • Hcrtr2 antibody
    • Hypocretin (orexin) receptor 2 antibody
    • Hypocretin receptor 2 antibody
    • Hypocretin receptor type 2 antibody
    • Orexin 2 receptor antibody
    • Orexin A/Orexin B receptor antibody
    • Orexin receptor 2 antibody
    • Orexin receptor type 2 antibody
    • Orexin type 2 receptor antibody
    • Ox-2-R antibody
    • Ox2-R antibody
    • Ox2r antibody
    • OX2R_HUMAN antibody
    • Type 2 hypocretin receptor antibody
    • Type 2 orexin receptor antibody
    see all

Anti-Orexin Receptor 2 antibody 画像

  • All lanes : Anti-Orexin Receptor 2 antibody (ab43445) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a His tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (His tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 32.5 kDa (why is the actual band size different from the predicted?)

Anti-Orexin Receptor 2 antibody (ab43445) 使用論文

This product has been referenced in:
  • Heydendael W  et al. Orexins/hypocretins act in the posterior paraventricular thalamic nucleus during repeated stress to regulate facilitation to novel stress. Endocrinology 152:4738-52 (2011). WB ; Rat . Read more (PubMed: 21971160) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"