製品の概要

  • 製品名Optiblot SDS Run Buffer (20X)
    Western blot buffer ihc 試薬 製品一覧
  • アプリケーション適用あり: SDS-PAGEmore details
  • 特記事項Optiblot SDS RunBuffer is optimised for use with Optiblot Precast Gels to provide the best running conditions and optimal sample resolution. Supplied in two volumes, 500ml or a in convenient 5 litre cube for high volume users. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr weights. Separation pattern is similar to that produced by a conventional MOPS buffer system.

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

製品の特性

  • 保存方法Store at room temperature.
  • バッファーpH: 8.30
    Constituents: 14% Tricine, 15% Tris, 0.95% Sodium bisulfite, 2% SDS
    Note: The pH should be between 8.2 and 8.3 @ 25°C. For Non-reduced samples (especially antibodies), omit the Sodium Bisulfite. For best results use low conductance ingredients to formulate the buffers. Do NOT use acid or base to adjust pH of buffers.
  • Concentration information loading...
  • 研究分野
  • 別名
    • SDS PAGE Western blot buffer
    • WB buffer
    • Western Blot

アプリケーション

Our Abpromise guarantee covers the use of ab119197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
SDS-PAGE Use at an assay dependent dilution.

プロトコール

Optiblot SDS Run Buffer (20X) (ab119197) 使用論文

ab119197 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119197.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"