Optiblot Prestained Marker (ab119210)

製品の概要

  • 製品名Optiblot Prestained Marker
    Prestained Protein Ladder ihc 試薬 製品一覧
  • アプリケーション適用あり: WB, SDS-PAGEmore details
  • 特記事項Optiblot Prestained Markers have been specifically formulated for use with Optiblot Gels and Buffers. Marker proteins are conjugated to dye molecules to show position before staining indicating how far your protein has migrated and to confirm the blotting efficiency without the need to stain your target protein.

    Optiblot Prestained Markers consist of 195, 142, 96, 71, 48, 33, 28, 22, 12 and 7.6 kDa standards. For additional convenience and ease of identification, the 71 and 28 kDa standards have been conjugated to an orange dye molecule.

    A load of only 4 µl Optiblot Pre-stained Markers is sufficient for a high intensity marker ladder (nearly 2.5x less loading than conventional ladders).

    The prestained markers are provided ready to use in 1x Optiblot sample buffer(ab119196). Recommended volumes to load for a 12 well comb are:

    To Monitor during run: 10 µl/well
    Visisble after blotting: 4 µl/well
    Visible after staining: 4 µl/well

    Optiblot gels must be used with Optiblot buffers.

    Buffer recipes are available in the protocol tab.

製品の特性

  • 別名
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder

アプリケーション

Our Abpromise guarantee covers the use of ab119210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
SDS-PAGE Use at an assay dependent concentration.

Optiblot Prestained Marker 画像

  • ab119210 separated on a 12%, 12 well Optiblot gel.

    Lane 1: Prior to staining with Optiblot blue (ab119211)
    Lane 2: After staining with Optiblot blue (ab119211)

Optiblot Prestained Marker (ab119210) 使用論文

ab119210 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. My colleague in the lab informed me that our optiblot range of protein molecular weight markers do contain His-tagged proteins. The markers ab115832 and ab48854 do not contain His-tagged proteins. I hope this helps, please ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"