Anti-OPALIN 抗体 (ab121425)
Key features and details
- Rabbit polyclonal to OPALIN
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-OPALIN antibody -
製品の詳細
Rabbit polyclonal to OPALIN -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
amino acids 55-141 (RRRSSIEAME ESDRPCEISE IDDNPKISEN PRRSPTHEKN TMGAQEAHIY VKTVAGSEEP VHDRYRPTIE MERRRGLWWL VPRLSLE) of Human OPALIN.
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ポジティブ・コントロール
- Human cerebellum tissue; Transfected HEK293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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特記事項 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ターゲット情報
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組織特異性
Brain specific. -
細胞内局在
Cell membrane. In the CNS, enriched in the myelin paranodal and inner loop membranes, but not that of the PNS. - Information by UniProt
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参照データベース
- Entrez Gene: 93377 Human
- SwissProt: Q96PE5 Human
- Unigene: 12449 Human
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別名
- HTMP10 antibody
- Oligodendrocytic myelin paranodal and inner loop protein antibody
- OPALI_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.
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All lanes : Anti-OPALIN antibody (ab121425) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab121425 は 2 報の論文で使用されています。
- Yang Y et al. Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis. Bioengineered 12:6643-6654 (2021). PubMed: 34516348
- Jäkel S et al. Altered human oligodendrocyte heterogeneity in multiple sclerosis. Nature 566:543-547 (2019). PubMed: 30747918