製品の概要

  • 製品名Anti-OPA1 antibody
    OPA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to OPA1
  • アプリケーション適用あり: IHC-FoFr, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Drosophila melanogaster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Rat OPA1.

    (Peptide available as ab42363.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human, Rat and Mouse Brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/200 - 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa).

Abcam recommends using milk as the blocking agent.

IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
  • 組織特異性Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
  • 関連疾患Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
    Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
  • 配列類似性Belongs to the dynamin family.
  • 翻訳後修飾PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
  • 細胞内局在Mitochondrion inner membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dynamin like 120 kDa protein antibody
    • Dynamin like 120 kDa protein, mitochondrial antibody
    • Dynamin-like 120 kDa protein, form S1 antibody
    • FLJ12460 antibody
    • Juvenile kjer type optic atrophy antibody
    • KIAA0567 antibody
    • KJER type antibody
    • Large GTP binding protein antibody
    • largeG antibody
    • MGM1 antibody
    • Mitochondrial dynamin like 120 kDa protein antibody
    • Mitochondrial dynamin like GTPase antibody
    • NPG antibody
    • NTG antibody
    • OAK antibody
    • OPA 1 antibody
    • opa1 antibody
    • OPA1 gene antibody
    • OPA1_HUMAN antibody
    • Optic atrophy 1 (autosomal dominant) antibody
    • OPTIC ATROPHY 1 antibody
    • Optic atrophy 1 gene protein antibody
    • Optic atrophy 1 homolog (human) antibody
    • Optic atrophy protein 1 antibody
    • Optic atrophy protein 1 homolog antibody
    see all

Anti-OPA1 antibody 画像

  • All lanes : Anti-OPA1 antibody (ab42364) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Anti-OPA1 antibody (ab42364) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 86 kDa (possible isoform).
    Although the predicted band size is ~111 kDa based on Swiss-prot data, bands of 92 kDa and 86 kDa has been previously observed. Investigative Ophthalmology & Visual Science, November 2005, Vol. 46, No. 11
  • OPA1 was immunoprecipitated using 0.5mg Rat Brain whole tissue lysate, 5µg of Rabbit polyclonal to OPA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab42364.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 92kDa: OPA1; non specific - 100kDa: We are unsure as to the identity of this extra band.

Anti-OPA1 antibody (ab42364) 使用論文

This product has been referenced in:
  • Norton M  et al. ROMO1 is an essential redox-dependent regulator of mitochondrial dynamics. Sci Signal 7:ra10 (2014). Read more (PubMed: 24473195) »
  • Gao J  et al. CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins. J Cell Biol 207:213-23 (2014). Read more (PubMed: 25349259) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Gel Running Conditions Reduced Denaturing (Bis-Tris 12%)
Loading amount 20 µg
Specification Peripheral Blood Mononuclear Cell
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Daniel Tyrrell

Verified customer

投稿 Nov 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Tissue sections (Brain, Hippocampus)
Specification Brain, Hippocampus
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Prof. Chichung Lie

Verified customer

投稿 Aug 01 2013

Thank you for contacting us. This antibody has not been tested for staining in cells, only in tissue sections. Typically antibodies that work in tissue sections do work in cells but we cannot guarantee it.

I hope this information is helpful to...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (retina)
Loading amount 30 µg
Specification retina
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"