製品の概要

  • 製品名Anti-ONECUT2 antibody
    ONECUT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ONECUT2
  • 特異性ab28466 recognises one cut domain family member 2 (ONECUT2)
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Zebrafish
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide derived from residues 274 - 323 of human ONECUT2.

    .

  • ポジティブ・コントロール
    • Jurkat cell lysate. Human kidney

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性ONECUT2 is a member of the transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. ONECUT2 participates in the network of transcription factors required for liver differentiation and metabolism.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • HGNC:8139 antibody
    • MGC120377 antibody
    • MGC120378 antibody
    • OC 2 antibody
    • OC2 antibody
    • One cut domain family member 2 antibody
    • ONECUT 2 antibody
    • ONECUT 2 homeodomain transcription factor antibody
    • ONECUT 2 protein antibody
    • ONECUT2 homeodomain transcription factor antibody
    • ONECUT2 protein antibody
    • Transcription factor ONECUT 2 antibody
    • Transcription factor ONECUT2 antibody
    see all

Anti-ONECUT2 antibody 画像

  • Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ONECUT2 expression detected in human kidney sections with 4-8ug/ml ab28466. The arrows indicate positive staining (epithelial cells of renal tubule). Magnification: 40X.
  • All lanes : Anti-ONECUT2 antibody (ab28466) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish liver homogenate (20ug)
    Lane 3 : Mouse liver homogenate (20ug)
    Lane 4 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-ONECUT2 antibody (ab28466) 使用論文

This product has been referenced in:
  • Bhatnagar S  et al. Increased microRNA-34c abundance in Alzheimer's disease circulating blood plasma. Front Mol Neurosci 7:2 (2014). WB ; Human . Read more (PubMed: 24550773) »
  • Dusing MR  et al. Onecut-2 knockout mice fail to thrive during early postnatal period and have altered patterns of gene expression in small intestine. Physiol Genomics 42:115-25 (2010). IHC-P ; Mouse . Read more (PubMed: 20354101) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Jul 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"